The accuracy of Enzyme Linked Immunosorbent Assay (ELISA) results is dependent on several factors. To get accurate results, the quality of the samples of antibodies, enzymes, or other biofluids should be maintained. And to do so, one should prevent contamination of the samples by maintaining a controlled temperature while performing the tests and using the right amount of substances like anticoagulants, enzyme inhibitors, etc. Since there is a high possibility of interference with the correct result, ELISA suppliers provide different ELISA kit quality and validation parameters. Let's learn how to be sure with ELISA test results while validating from different ELISA kit suppliers in India.
Factors to consider while verifying the results from different ELISA kit suppliers
Always opt for kits that have passed the standard sensitivity, specificity, and reproducibility. Quality control measures, like batch-to-batch consistency, can indicate reliable results that ensure the commitment of ELISA kit manufacturers in India. The following are some factors to consider.
Look into the sensitivity of the kit
Also known as the analytical sensitivity or limit of detection, it should be reported as per the industry standards by ELISA manufacturers. It is also referred as to the lowest level of analyte that can be distinguished from the background. Sensitivity is obtained by adding two standard deviations to the mean O.D. obtained from replicates of the zero standard.
Check the specificity
Testing is done to prevent cross-reactivity that can lead to false positive results or inaccurate quantification. To ensure that only the desired analyte is recognized, a panel of other substances is tested for cross-reactivity.
Go through manufacturer’s regulatory standards
The manufacturers of the Elisa kit must follow a consistent manufacturing process that includes thorough documentation and record-keeping, personnel training, quality control procedures, facility and equipment maintenance, validation of critical processes, raw material control, traceability, environmental monitoring, change control, and compliance with regulatory standards.
Evaluate the precision
Precision evaluation of ELISA kits from various ELISA kit distributor helps you to determine that you are likely to obtain consistent results from using the kit. The ELSA kit must have a description with an Average Intra Assay CV < 10 % and an Average Inter Assay CV < 10%. Intra-assay precision tests multiple samples in a replicate on the same plate. Inter-assay precision tests multiple samples on different days and different plates.
Check guidelines for parameters
ELISA kit suppliers provide parameter data like recovery and linearity in their product specifications. For example, while comparing ELISA kits from different suppliers you must consider that the recovery should be close to 100%.
Analyze statistical validation
When running an ELISA test, assign the values of the unknown samples about the standard curve. If the samples have been diluted, you must multiply the concentration from the standard curve by the dilution factor. You should always run ELISA samples in duplicate or triplicate to provide enough data for statistical validation. This ensures the accuracy of the test.
Conclusion
Validating the results based on the discussed parameters minimizes the chance of inaccuracy. These ELISA kits are developed in such a manner that all the components work together and perform perfectly to give accurate results. Universal Biotechnology’s ELISA kits meet all the standards. Their products are also available at reasonable ELISA kits price.
